Chromatographic separation of brain lipids. 2. Ethanolamine-containing phospholipids

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Quantitative gas-liquid chromatographic analysis of ethanolamine, monomethyl ethanolamine, and dimethyl ethanolamine from lipids.

A method for the separation and quantitative estimation of ethanolamine, dimethyl ethanolamine, and monomethyl ethanolamine derived from lipids is described. After acid hydrolysis of the lipids, the bases are extracted from the neutralized hydrolysate with t-butonol and separated by gas-liquid chromatography.

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Molecular species of neutral phospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), were resolved by reversed-phase high-performance liquid chromatography (HPLC) using mobile phases of acetonitrile-methanol-water containing tetraalkylammonium phosphates (TAAPs). Competitive interactions of TAAPs and analyte solutes with a reversed-phase HPLC column resulted in reduced retent...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1961

ISSN: 0006-2936

DOI: 10.1042/bj0800557